Human VPS39 ELISA Kit (HUFI05703)

VPS39 (vacuolar protein sorting 39, also known as TULARA) is a core subunit of the HOPS tethering complex that orchestrates late endosome and lysosome fusion. The Assay Genie Human VPS39 ELISA Kit enables quantitative measurement of this protein in human samples, supporting research into autophagosome maturation, Golgi-to-endosome trafficking, and the maintenance of cellular degradative pathways that have been linked to neurodegenerative disease, innate immune signaling, and metabolic disorders. Investigators probing how the HOPS complex coordinates with RAB7-GTP and SNARE machinery during cargo delivery to the lysosome rely on VPS39 readouts to track endolysosomal flux in cell-based and tissue-based studies.

Configured for human samples, the assay runs on a standard microplate workflow and is supplied in a 96 Assays kit format. The kit is intended for research use only in laboratory settings studying autophagy regulation, membrane trafficking biology, and the molecular mechanisms underlying lysosomal storage disorders. Each kit provides the essential components required for sample dilution, standard curve preparation, and quantitative readout of VPS39 levels in extracted cellular and tissue-derived specimens.

Storage and handling: unopened components should be kept refrigerated and allowed to equilibrate to room temperature before use. Substrate reagents must be protected from light, and reconstituted materials should be aliquoted to limit repeated freeze-thaw cycles if long-term storage is required. Standard incubation, wash, and development steps apply, with absorbance readings captured within the linear working range of the standard curve generated for each run.