Human Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin) ELISA Kit

The Assay Genie vaspin immunoassay provides quantitative measurement of visceral adipose tissue-derived serpin in human research specimens. Vaspin (SERPINA12) is an adipokine secreted predominantly by visceral white adipose tissue, with circulating levels frequently investigated in studies of obesity, insulin resistance, type 2 diabetes mellitus, and metabolic syndrome. Researchers use this kit to evaluate vaspin as a candidate biomarker in adipose biology, glucose homeostasis, and low-grade inflammatory signaling.

The assay is supplied in a microplate format with reagents sufficient for 96 determinations, supporting moderate-throughput screening of clinical cohorts and cell culture supernatants. Standard curves are generated in parallel with unknowns, and absorbance readings are taken on a standard plate reader. Each kit includes a pre-coated plate, assay diluent, detection antibody, recombinant standard, wash buffer, and substrate solution, along with a detailed protocol optimized for research use only.

Sample handling guidance emphasizes that whole blood should be processed promptly, with clarified liquid fractions aliquoted and stored frozen to preserve analyte integrity across multiple freeze-thaw cycles. Researchers studying tissue expression can prepare homogenates from fresh or snap-frozen adipose biopsies, normalizing total protein concentration before loading. By enabling reproducible quantification of this adipocytokine, the Assay Genie kit supports cross-sectional and longitudinal study designs in endocrinology and metabolic disease research.