Human VIP/Vasoactive intestinal peptide ELISA Kit

From Assay Genie, this human VIP (Vasoactive intestinal peptide) ELISA kit is a colorimetric Sandwich ELISA using a double-antibody format, designed for the quantitative measurement of VIP in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. VIP is a neuropeptide involved in regulating immune responses, neurotransmission, and smooth muscle relaxation, and its dysregulation has been linked to inflammatory bowel disease, asthma, and neurological disorders.

The assay achieves a sensitivity of 0.188 pg/mL with a measuring range of 0.313-20 pg/mL. Components are stored at 2-8°C for 12 months. Reported linearity ranges from 82% to 105% across serum, EDTA plasma, and heparin plasma at dilutions of 1:2, 1:4, and 1:8, while recovery averages 95% in those matrices. The procedure uses a TMB substrate with absorbance measured at 450 nm.

The kit is intended for cell biology research, with the analyte referenced under UniProt ID P01282 and listed under the synonyms VIP, vasoactive intestinal peptide, VIP peptides, and PHM27.