Human Vimentin PharmaGenie ELISA Kit (SBRS1231)

Vimentin is a 57 kDa type III intermediate filament protein that serves as a canonical marker of mesenchymal lineage and epithelial-mesenchymal transition (EMT). This Assay Genie immunoassay enables quantitative measurement of vimentin in research samples, supporting studies in oncology, fibrosis, wound healing, and cell migration.

The kit includes a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP reagent, recombinant standards, and assay buffer sufficient for the full plate format. Researchers typically run the procedure in roughly 3 to 4 hours at room temperature, with absorbance read on a standard plate reader.

Application areas include biomarker profiling for circulating tumor cells, characterization of fibroblast activation in fibrotic disease models, and validation of EMT status in cell culture models. The assay is intended for research use only and is not validated for diagnostic procedures.