Human Vigilin (HDLBP) ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed to measure human Vigilin (HDLBP) in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The kit reacts specifically with natural and recombinant human Vigilin and is intended for research use only, supporting cardiovascular research applications. Vigilin is implicated in cell sterol metabolism, where it may function to protect cells from over-accumulation of cholesterol.

Performance characteristics include a detection range of 1.56-100 ng/mL and a sensitivity of 0.23 ng/mL. Intra-assay CV is reported at 4.3% and inter-assay CV at 8.7%. Linearity in serum (N=5) spans 97-115% across dilutions 1:2 to 1:16, with EDTA plasma at 90-111% and heparin plasma at 91-116% over the same dilution range. Spike-and-recovery performance shows an average of 103% (range 97-109%) in serum and an average of 105% (range 99-111%) in plasma.

The target protein is associated with the cytoplasm and nucleus, and the UniProt entry is Q00341 (NCBI Gene ID 3069). Listed synonyms include high density lipoprotein-binding protein, HDL-binding protein, HBP, and VGL, with a reported molecular weight of 1268.