Human VG5Q/AGGF1 ELISA Kit

Designed for cardiovascular research, this Assay Genie ELISA kit enables accurate quantification of VG5Q (AGGF1) in human serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. VG5Q (AGGF1) is a protein linked to angiogenesis — the formation of new blood vessels from existing ones — and has been implicated in conditions such as cancer and cardiovascular disorders. The kit uses a sandwich ELISA format with double-antibody detection and a colorimetric read-out, providing a straightforward protocol for studies of blood vessel development and related disease mechanisms.

Quantitative performance is characterised by a sensitivity of 18.75 pg/mL and an assay range of 31.25–2000 pg/mL. Reactivity is confirmed for human samples, and the kit is stored at 2–8°C for up to 12 months. The provided protocol covers reagent and plate preparation, primary incubation at 37°C for 90 minutes, biotinylated detection antibody binding, HRP-Streptavidin (SABC) binding, TMB colour development in the dark for 10–20 minutes, and absorbance measurement at 450 nm after adding the stop solution.

Validation data reported by Assay Genie include linearity across dilutions (1:2, 1:4 and 1:8) for serum (86–98%, 87–97%, 80–98%), EDTA plasma (85–97%, 83–100%, 81–99%) and heparin plasma (85–100%, 85–100%, 81–92%). Spike-and-recovery testing yielded ranges of 85–105% (average 93%) for serum, 87–101% (average 95%) for EDTA plasma and 85–96% (average 90%) for heparin plasma.