Human VEGF-D PharmaGenie ELISA Kit (SBRS1227)

This human VEGF-D immunoassay from Assay Genie is intended for research use only and supports quantitative measurement of Vascular Endothelial Growth Factor D in cell culture supernatant, plasma, and serum specimens. VEGF-D is a secreted ligand of the VEGF family that binds VEGFR-2 and VEGFR-3, making it a useful biomarker in studies of tumor lymphangiogenesis, lymphatic metastasis, and angiogenic signaling pathways. Each kit provides reagents sufficient for 96 determinations run on a standard microplate reader, making it suited to mid-throughput screening of conditioned media from lymphatic endothelial or tumor cell cultures as well as clinical research specimens. The protocol uses a two-site capture-and-detection configuration with a colorimetric readout, allowing laboratories to integrate VEGF-D quantification alongside related angiogenic factor panels without specialized instrumentation. Researchers studying lymph node metastasis, primary lymphedema, or VEGFR-3 pathway modulation will find this assay useful for tracking changes in VEGF-D levels in response to genetic, pharmacological, or environmental interventions in vitro and in vivo.