Human VAT1 (Vesicle amine transport 1) ELISA Kit (HUFI05734)

Vesicle Amine Transport Protein 1 (VAT1) is a multi-pass transmembrane protein localized to secretory vesicles and the endoplasmic reticulum, where it participates in the storage and release of biogenic amines. The Assay Genie Human VAT1 ELISA Kit provides a quantitative immunoassay for measuring this target in human research specimens, supporting work in vesicle trafficking, monoamine neurotransmitter packaging, and pancreatic islet biology, including β-cell dysfunction associated with type 2 diabetes.

The microplate kit accepts validated human serum specimens and EDTA-plasma collections as primary input matrices. Investigators commonly pair this ELISA with cell culture supernatant or tissue lysate prepared in-house, diluting specimens into the kit's standard buffer range. All reagents are intended for research use only and are not validated for clinical diagnostic use.

The kit ships with pre-coated microwell strips, a biotinylated detection antibody, streptavidin-HRP conjugate, a calibrated standard set, wash buffer, and chromogenic substrate sufficient for the full plate layout. Reagents should be stored refrigerated upon arrival and equilibrated to room temperature before each run; each lot is quality-controlled against reference standards to confirm reproducibility across the dynamic range of the standard curve.