Human VANGL1 ELISA Kit (HUFI05731)

The Human VANGL1 ELISA Kit from Assay Genie targets Vang-Like Protein 1, a four-pass transmembrane component of the non-canonical Wnt/planar cell polarity (PCP) signaling pathway. VANGL1 plays a documented role in neural tube closure, oriented cell division during gastrulation, and epithelial morphogenesis, making the kit useful for developmental biology, oncology, and PCP-pathway research.

The assay uses a human-specific capture-and-detection antibody pair with no significant cross-reactivity against mouse Vangl1 or rat Vangl1, supporting clean quantification of human VANGL1 in serum and plasma samples. Each microplate kit ships with pre-coated wells, lyophilized standards, biotinylated detection antibody, streptavidin-HRP conjugate, TMB substrate, and wash buffer. Store unopened components at 2 to 8 degrees C, protect the substrate from light, and avoid freeze-thaw cycles on diluted working solutions.

Typical workflows run at room temperature using a 100 uL sample volume and a standard sandwich ELISA protocol read at 450 nm on a microplate reader. Common research applications include profiling VANGL1 expression in colorectal, breast, and hepatocellular carcinoma cohorts, investigating neural tube defect genetics such as craniorachischisis, and screening small-molecule modulators of PCP signaling in cell-based studies.