Human USP49 ELISA Kit (HUFI05741)

This Assay Genie kit measures USP49 (Ubiquitin Specific Peptidase 49), a deubiquitinating enzyme that removes ubiquitin from histone H2B and modulates pre-mRNA splicing through spliceosome interactions. Researchers studying chromatin dynamics, epigenetic regulation, and cancer pathways use this microplate-based immunoassay to quantify USP49 protein in human specimens. The assay incorporates pre-coated capture wells, biotinylated detection antibody, and streptavidin-HRP, with a chromogenic readout measured spectrophotometrically. Each kit contains standards for generating a multi-point calibration curve, and reagents are supplied in individual vials for single-use preparation. Storage at 4°C is recommended to preserve antibody activity through the kit's shelf life. The protocol accommodates routine laboratory workflows with a total incubation time of approximately 3.5 hours. For research use only; not for diagnostic procedures.