Human USP33 (Ubiquitin carboxyl-terminal hydrolase 33) ELISA Kit (HUFI05758)

The Human USP33 ELISA Kit from Assay Genie quantifies Ubiquitin carboxyl-terminal hydrolase 33, a deubiquitinating enzyme involved in centrosome biogenesis, mitotic checkpoint control, and receptor endocytosis. USP33 modulates Robo1 axon guidance receptor stability and counteracts the E3 ligase involved in beta2-adrenergic receptor downregulation, making this assay useful in cancer biology, neuroscience, and GPCR signaling research.

Each microplate-based kit includes pre-coated wells, lyophilized recombinant standards, biotinylated detection antibody, streptavidin-HRP, wash concentrate, and TMB substrate. The protocol calls for 100 uL of diluted sample per well, followed by incubation, washing, and color development measured by absorbance. Store unopened reagents refrigerated at 4 degrees C and protect the plate from moisture; allow all components to reach room temperature before use. For research use only.