Human USP3 ELISA Kit (HUFI05756)

The Ubiquitin-Specific Protease 3 (USP3) quantification kit from Assay Genie provides a sensitive immunoassay platform for measuring human USP3 protein in research samples. USP3 is a deubiquitinating enzyme that cleaves ubiquitin from histone H2A and other chromatin-associated substrates, with established roles in DNA damage response, genome stability, and cell cycle regulation. Investigators working on post-translational modification pathways, BRCA1-mediated chromatin remodeling, and oncogenic signaling in hematopoietic and solid tumor models routinely apply this kit to in vitro research workflows exploring the ubiquitin-proteasome system.

Each 96 Assays kit supplies pre-coated plate strips, recombinant USP3 standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, and TMB substrate. The protocol involves sequential incubation and wash steps read on a conventional microplate reader, with USP3 concentrations calculated against the included standard curve. Reconstituted reagents and unused strip wells remain stable for repeated use within the kit shelf life when handled appropriately.

Storage: refrigerate kit components upon arrival and protect from light; allow all reagents to reach room temperature before initiating the run. Avoid repeated freeze-thaw cycles of the standard and detection antibody. Components are intended for research use only and are not approved for diagnostic procedures. The format supports biomarker validation in clinical research cohorts, mechanistic investigation of deubiquitinase activity, and screening campaigns for small-molecule modulators of the ubiquitin pathway.