Human USP16 ELISA Kit (HUFI05762)

The Human USP16 ELISA Kit from Assay Genie is supplied for research use only and enables quantitative measurement of ubiquitin-specific peptidase 16 (USP16), a chromatin-associated deubiquitinase that removes ubiquitin from histone H2A and regulates gene expression, mitosis, and the DNA damage response. Altered USP16 activity has been reported in multiple myeloma, breast cancer, and other malignancies, supporting applications in oncology biomarker research, epigenetics, stem cell biology, and cell signaling pathway studies.

Each kit contains a pre-coated microplate, capture and detection antibodies, recombinant standards, wash buffer, substrate solution, and stop reagent. Researchers commonly use the assay to profile USP16 abundance in biological samples, monitor changes following gene knockdown or small-molecule inhibitor treatment, and validate targets identified in chromatin remodeling or proteasome studies.

Store unopened components refrigerated and protect antibody vials from light until first use. Allow all reagents to equilibrate to room temperature before initiating the protocol, and refer to the enclosed manual for recommended sample dilution factors, incubation times, and standard curve preparation to ensure reproducible quantification across experiments.