Human Urotensin 2 ELISA Kit

This Sandwich ELISA kit from Assay Genie is designed for the quantitative measurement of Urotensin 2 in human samples. Using a double-antibody sandwich immunoassay format with colorimetric detection, it supports research into cardiovascular and vascular biology.

The kit offers a sensitivity of 9.375 pg/mL and a standard range of 15.625–1000 pg/mL. It is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types, with all components stable at 2–8°C for 12 months. Reported linearity and recovery performance are documented across serum, EDTA plasma, and heparin plasma at multiple dilutions.

The workflow includes equilibrating reagents and TMB substrate, loading standards and samples onto pre-coated wells, primary incubation at 37°C, biotinylated detection antibody binding, HRP-streptavidin incubation, color development with TMB substrate in the dark, and absorbance measurement at 450 nm after adding stop solution. Urotensin 2 is a vasoactive peptide hormone involved in regulating blood pressure and vascular tone, and its dysregulation has been associated with cardiovascular conditions, making it a relevant biomarker for cardiovascular research.