Human Uridine phosphorylase 1 (UPP1) ELISA Kit

A high-quality Sandwich ELISA from Assay Genie designed for the quantitative measurement of human Uridine phosphorylase 1 (UPP1) in human biological samples. The colorimetric assay is intended for research use only, supporting investigations in fields such as cancer and nucleotide metabolism.

Validated sample types include serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, with reactivity confirmed for human samples. The kit detects natural and recombinant human Uridine phosphorylase 1 and has a detection range of 31.2-2000pg/mL with a sensitivity of 15.64pg/mL. Reported intra-assay CV is 5.0% and inter-assay CV is 8.3%. Recovery is documented at an average of 92% (range 86-98%) for serum and 94% (range 88-100%) for plasma.

UPP1 is a homodimeric enzyme (UniProt Q16831) that catalyses the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate, playing a role in pyrimidine nucleoside salvage. Kit components are supplied for storage at -20°C or 4°C depending on the reagent, with shipping at +4°C.