Human UPF3B ELISA Kit (HUFI05789)

The Human UPF3B enzyme-linked immunosorbent assay from Assay Genie enables quantitative measurement of upstream frameshift factor 3B, a core component of the exon junction complex that drives nonsense-mediated mRNA decay in eukaryotic cells. The assay is reactive with human UPF3B protein and is intended for laboratory research applications in molecular biology, neuroscience, and clinical research.

UPF3B sits on the X chromosome and has been linked through genetic studies to X-linked intellectual disability, autism spectrum disorder, and schizophrenia, making quantitative protein measurement valuable for investigations of post-transcriptional gene regulation and the molecular consequences of UPF3B loss-of-function mutations in patient-derived samples and disease model systems.

Provided as a complete kit of reagents, the assay is configured for 96 Assays determinations and uses a solid-phase capture antibody pre-coated onto a microplate. Researchers carry out incubation steps at room temperature, develop the signal, and read absorbance on a standard plate reader. Components should be stored according to the insert to preserve antibody activity, and unopened kits remain stable through the printed expiration date. For research use only.