Human uPAR PharmaGenie ELISA Kit (SBRS1219)

The Assay Genie uPAR (urokinase plasminogen activator receptor, PLAUR) immunoassay enables quantitative measurement of soluble uPAR from cell culture supernatant, plasma, or serum-derived specimens. uPAR is a GPI-anchored glycoprotein that localizes urokinase plasminogen activator at the cell surface, where it drives extracellular matrix degradation, cell migration, and signal transduction events central to tumor invasion and inflammation research.

Wells of the microplate are pre-coated with a human-specific capture antibody directed against PLAUR. Reconstitute standards and working reagents per the supplied protocol, add samples alongside a biotinylated detection antibody, then introduce streptavidin-HRP. After washing, TMB substrate develops a measurable absorbance change that is quenched with stop solution before plate reading.

Store the unopened kit refrigerated and protect from light. Investigators studying cancer metastasis, epithelial-mesenchymal transition, fibrinolysis, or in vitro urokinase pathway signaling rely on this assay to track PLAUR expression across experimental conditions.