Human Uncharacterized protein C13orf33 (C13orf33) ELISA Kit

Assay Genie offers a Sandwich ELISA Kit designed for the precise quantification of human Uncharacterized protein C13orf33 in serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids. C13orf33, also known as chromosome 13 open reading frame 33 or mesenteric estrogen-dependent adipogenesis protein (MEDAG), is implicated in processes that promote adipocyte differentiation, lipid accumulation, and glucose uptake in mature adipocytes, making it a valuable biomarker for investigating its functions and implications in different diseases and conditions.

The kit is calibrated to a detection range of 78-5000pg/mL with a sensitivity of 33pg/mL. Reproducibility is supported by an intra-assay CV of 6.3% and an inter-assay CV of 9.6%. Linearity has been validated across serum, EDTA plasma, and heparin plasma at dilutions of 1:2, 1:4, 1:8, and 1:16, with serum recovery averaging 100% (range 94-106%) and plasma recovery averaging 102% (range 96-108%).

Specificity is directed against the natural and recombinant human mesenteric estrogen-dependent adipogenesis protein. The associated UniProt entry is Q5VYS4, mapping to chromosomal location 13q12.3, with a reported molecular weight of 17,194 Da and cytoplasmic subcellular localisation. The kit is intended for research use only.