Human UHRF1 ELISA Kit (HUFI05798)

Epigenetic regulation research and cancer biomarker discovery both benefit from sensitive quantification of UHRF1, a nuclear protein that maintains CpG methylation through DNA replication. This microplate immunoassay kit from Assay Genie delivers quantitative measurement of human UHRF1 across 96 individual determinations, with all reagents supplied in a single pack.

UHRF1 (Ubiquitin-like with PHD and RING finger domain 1) recruits DNMT1 to hemimethylated CpG sites during S-phase, sustaining inheritance of DNA methylation patterns. Aberrant UHRF1 expression is documented in hepatocellular carcinoma, breast cancer, lung adenocarcinoma, and gastric tumors, where elevated levels correlate with tumor suppressor silencing and poor prognosis. Investigators studying hepatitis B virus replication, where UHRF1 supports covalently closed circular DNA stability, also rely on accurate protein measurements.

Each kit provides pre-coated wells, biotinylated detection antibody, recombinant UHRF1 standards, streptavidin-HRP conjugate, wash buffer, substrate solution, and stop reagent, ready for use with human plasma and serum specimens. The protocol follows standard ELISA practice: add sample and standard, incubate, wash, detect with TMB substrate, and read absorbance on a microplate reader. Intended for research use only, the assay integrates into chromatin immunoprecipitation follow-up, methylation profiling studies, and cell-line screening panels. Store kit components refrigerated and protected from light between uses. Avoid repeated freeze-thaw cycles to preserve activity through the stated shelf life.