Human UDP-glucuronosyltransferase 1-6 (UGT1A6) ELISA Kit

A sandwich ELISA kit from Assay Genie designed for the quantitative detection of human UDP-glucuronosyltransferase 1-6 (UGT1A6) in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The assay uses a colorimetric detection method and is intended for research use only, supporting studies of glucuronidation and xenobiotic metabolism.

Reported performance characteristics include a detection range of 0.156-10ng/mL and a sensitivity of 0.067ng/ml, with intra-assay CV of 7.3% and inter-assay CV of 9.8%. Specificity is reported for natural and recombinant human UGT1A6. Mean serum recovery is 101% (range 95-107%) and plasma recovery 103% (range 97-109%). UGT1A6 (UDPGT) is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds; the isoform has specificity for phenols, while isoform 3 lacks transferase activity and acts as a negative regulator of isoform 1.

Within the broader UDP-glycosyltransferase family, the enzyme is localised to the endoplasmic reticulum membrane as a single-pass integral membrane protein and is catalogued under UniProt P19224. Listed synonyms include phenol-metabolizing UDP-glucuronosyltransferase, UDP-glucuronosyltransferase 1-F, UDP-glucuronosyltransferase 1A6, UGT-1F, UDPGT 1-6, GNT1 and UGT1. The product falls within the metabolism research area.