Human Ubiquitin Specific Peptidase 14 (USP14) ELISA Kit

Configured for 96 assays and in vitro research use only, this Assay Genie quantitative immunoassay targets human Ubiquitin-Specific Protease 14 (USP14), a 19S regulatory-particle-associated deubiquitinating enzyme that trims ubiquitin chains from substrate proteins at the proteasome. By monitoring this activity, researchers investigate proteostasis, neurodegeneration pathways, cancer cell signaling, and related processes where altered ubiquitin-proteasome flux is of interest.

The kit contains a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, recombinant USP14 standards, and assay diluents. Samples are prepared from tissue homogenates, and the assay relies on a horseradish peroxidase-TMB reaction with an absorbance readout. To preserve reagent activity, unopened components are refrigerated at 4 degrees C; reconstituted standards and other working solutions should be aliquoted and stored frozen at -20 degrees C, with limited freeze-thaw exposure to maintain stability over approximately one month.

Standard curves are generated from recombinant human USP14 protein, with sample concentrations interpolated using a four-parameter logistic fit. Investigators adapt the assay to evaluate USP14 levels in response to proteasome inhibitors, oxidative stress, or genetic manipulation of upstream regulators. Each plate includes blank and internal control wells to support intra-assay reproducibility. Complete protocol documentation, technical support, and SDS information are provided by Assay Genie.