Human Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2) ELISA Kit

Mitochondrial respiratory chain research demands quantitative tools capable of resolving subtle changes in complex III subunit abundance. This Assay Genie ELISA kit targets Ubiquinol-Cytochrome C Reductase Core Protein II (UQCRC2), the catalytic core subunit of mitochondrial complex III embedded in the inner mitochondrial membrane. UQCRC2 contributes to electron transfer from ubiquinol to cytochrome c and is implicated in mitochondrial disorders, neurodegeneration studies, and cancer metabolism investigations.

The microplate format uses an absorbance-based readout on tissue homogenates and human-derived research samples, supporting measurement of UQCRC2 protein levels across cell and tissue models. Researchers studying oxidative phosphorylation, mitochondrial dysfunction biomarkers, or biogenesis pathways can integrate this kit into western blot validation workflows, cell culture experiments, and in vitro mitochondrial assays.

Kit components should be stored refrigerated, protected from light, until the expiration date printed on the outer label. Each kit is supplied as a box of pre-coated wells, detection reagents, and standards sufficient for 96 individual determinations. The Assay Genie platform provides a protocol booklet with step-by-step sample preparation guidance for homogenization, dilution series construction, and standard curve generation.

For investigations focused on complex III assembly, UQCRC2 expression profiling in patient-derived fibroblasts, or comparative studies between diseased and healthy tissue, this kit delivers a focused protein-level readout complementing transcriptomic and proteomic datasets.