Human UBE2S ELISA Kit (HUFI05834)

Quantitative detection of ubiquitin-conjugating enzyme E2 S (UBE2S) in human specimens supports oncology and cell-cycle research programs investigating APC/C-dependent proteolysis and mitotic exit. The kit from Assay Genie is configured for in vitro research use only and provides the core reagents needed to run a standard immunometric assay in a microplate workflow, with all buffers, standards, and conjugate components supplied ready to use. UBE2S catalyzes K11-linked polyubiquitin chain formation on cyclin B and securin, and altered expression has been documented in non-small cell lung cancer, hepatocellular carcinoma, and glioblastoma tissue, making quantitative measurements a useful avenue for biomarker discovery projects. Each kit contains a pre-coated plate, detection antibody, standard diluent, wash concentrate, substrate solution, and stop reagent, packaged for bench-top use with straightforward well-by-well processing. Researchers studying the ubiquitin-proteasome system, anaphase-promoting complex activity, or tumor proliferation markers can apply this reagent to longitudinal sample screening and disease-progression studies. The protocol is intended for laboratory professionals familiar with quantitative immunoassay procedures and standard curve generation by serial dilution.