Human UBE2K ELISA Kit (HUFI05839)

The UBE2K protein (Ubiquitin-Conjugating Enzyme E2 K), also known as HIP-2 or UBC1, is a key E2 ubiquitin-conjugating enzyme that catalyzes ubiquitin chain elongation within the ubiquitination cascade. This Assay Genie ELISA Kit enables quantitative measurement of UBE2K levels in human samples, supporting research into proteasome-mediated protein degradation, post-translational modification pathways, and neurodegenerative disease mechanisms including studies of Huntington's, Alzheimer's, and Parkinson's disease pathology. The kit is supplied as a 96 Assays format with pre-coated microplate wells, calibration standards, detection antibody, substrate solution, stop reagent, and wash buffer sufficient for a full plate run.

Researchers can analyze patient-derived sample fractions including serum fractions, plasma preparations, or cell lysates, to investigate UBE2K expression changes associated with cellular stress, apoptosis regulation, or cancer progression. Standard laboratory absorbance measurement provides reliable quantification following the included protocol. All reagents should be stored refrigerated between uses; bring components to room temperature before running the assay, and avoid repeated freeze-thaw cycles of prepared aliquots. Each kit is intended for research use only and supports experimental work in molecular biology, cell biology, and biochemistry laboratories exploring ubiquitin-proteasome system function and protein homeostasis.