Human UBE2I/SUMO-conjugating enzyme UBC9 ELISA Kit

From Assay Genie, this sandwich ELISA kit uses a double-antibody configuration for the in vitro quantitative determination of human UBE2I (SUMO-conjugating enzyme UBC9) in serum, plasma, cell culture supernatants and other biological fluids. UBE2I, also known as UBC9, functions as a SUMO-conjugating enzyme with a central role in protein sumoylation, a pathway implicated in DNA repair, transcriptional regulation and cell cycle progression.

Validation data provided by the manufacturer include spiked recovery of 89-103% (average 96%) in serum, 91-102% (average 97%) in EDTA plasma, and 85-103% (average 94%) in UFH plasma. Linearity upon serial dilution was demonstrated at 85-105% (serum, 1:2), 88-100% (serum, 1:4) and 88-105% (serum, 1:8), with EDTA plasma at 83-99% (1:2), 89-100% (1:4) and 87-97% (1:8) and UFH plasma at 80-100% (1:2), 80-98% (1:4) and 87-100% (1:8). Intra-assay precision is reported as CV

UBE2I/UBC9 is relevant to research areas including the cell cycle, and its dysregulation has been linked in published literature to cancer, neurodegenerative disorders and viral infections, making it a target of interest for therapeutic studies.