Human UBE2D3 ELISA Kit (HUFI05854)

Quantitative measurement of endogenous UBE2D3 levels in human specimens is achieved through this immunoassay kit from Assay Genie, developed to support research into ubiquitin-proteasome pathway function and protein homeostasis. UBE2D3 (Ubiquitin-Conjugating Enzyme E2D 3) is an E2 conjugating enzyme that partners with E1 activating enzymes and diverse E3 ligases to transfer activated ubiquitin onto substrate proteins, influencing cell cycle progression, DNA damage repair, NF-kB signaling, and innate immune responses.

The kit includes a pre-coated microplate, lyophilized recombinant protein standards, biotinylated detection antibody, streptavidin-HRP conjugate, concentrated wash buffer, assay diluent, and TMB substrate for endpoint absorbance reading. Reagents are supplied for 96 determinations per plate. Researchers apply this UBE2D3 ELISA to investigate expression changes in cancer cell lines, primary tissue samples, and peripheral blood specimens, and to screen small molecules that modulate ubiquitination activity in cell-based experiments and in vitro assays.

Storage recommendations: keep unopened components refrigerated and protect from light; bring all reagents to room temperature before use, and avoid repeated freeze-thaw cycles of diluted standards. The kit is supplied in a single-use format with components packed in individual vials for assay setup. For research use only.