Human Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1) ELISA Kit

Endothelial cell biology, vascular development, and angiogenic signaling are central research areas where quantitative measurement of the Tie1 receptor supports mechanistic and biomarker studies. This Assay Genie immunoassay is configured to measure human Tie1 protein levels across multiple research sample matrices and is applicable to cardiovascular, oncology, and inflammation research programs. The kit applies an absorbance-based readout on a standard microplate reader and ships as a 96-test plate configuration that includes pre-coated wells, a calibrated standard curve set, biotinylated detection antibody, streptavidin-HRP conjugate, substrate solution, stop reagent, and wash buffer. Researchers can apply the kit to cell culture experiments, conditioned media analysis, tissue lysate investigations, and other endothelial-focused workflows, supporting biomarker discovery in inflammatory, neovascular, and tumor vascular studies. A detailed step-by-step protocol accompanies each kit, describing reagent preparation, working dilutions, assay procedure, and data analysis guidance. The product is intended for research use only and is not validated for diagnostic or clinical decision-making.