Human Tyrosine Hydroxylase ELISA Kit

From Assay Genie, this Human Tyrosine Hydroxylase ELISA Kit is a Sandwich ELISA built on a double-antibody format for the quantitative measurement of tyrosine hydroxylase in human samples. The kit is compatible with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Assay performance includes a sensitivity of 0.188 ng/mL and a working range of 0.313–20 ng/mL. Reagents are stored at 2–8°C and remain stable for 12 months. Reported linearity across serum, EDTA plasma and heparin plasma at 1:2, 1:4 and 1:8 dilutions spans 82–105%, and recovery across the same matrices spans 86–105%. The procedure uses a TMB substrate with absorbance read at 450 nm.

According to Assay Genie, tyrosine hydroxylase is a key enzyme involved in the synthesis of neurotransmitters such as dopamine, norepinephrine and epinephrine, and dysregulation of the protein has been linked to neurological disorders including Parkinson's disease and schizophrenia. The kit is intended for research applications within the metabolism area.