Human Type III iodothyronine deiodinase (DIO3) ELISA Kit

From Assay Genie, this Sandwich ELISA kit is designed for the quantitative measurement of human Type III iodothyronine deiodinase (DIO3). The assay targets an enzyme central to thyroid hormone regulation and metabolism, and research using this kit has explored DIO3 dysregulation in connection with thyroid disorders, diabetes, and broader metabolic conditions, where it may serve as a valuable biomarker and route to potential therapeutic interventions.

Reported performance includes a detection range of 0.312-20ng/mL and a sensitivity of 0.11ng/mL. Precision values are 4.5% intra-assay CV and 8.2% inter-assay CV. Spike-and-recovery testing gave an average of 86% (range 80-92%) for serum and 88% (range 82-94%) for plasma.

The kit is validated for serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, with stated specificity for natural and recombinant human Thyroxine 5-deiodinase. For research use only.