Human Type II iodothyronine deiodinase (DIO2) ELISA Kit

Manufactured by Assay Genie, this competitive ELISA kit enables quantitative measurement of human Type II iodothyronine deiodinase (DIO2), a selenoprotein responsible for the deiodination of T4 into the active thyroid hormone T3. The assay provides high sensitivity and specificity for both natural and recombinant human DIO2, supporting research into thyroid function, metabolism, and associated disorders.

Performance specifications include a detection range of 0.156–10 ng/mL and a sensitivity of 0.078 ng/mL. Intra-assay CV is 5.1% and inter-assay CV is 8.9%. Linearity has been demonstrated in serum, EDTA plasma, and heparin plasma across dilutions from 1:2 to 1:16. Average recovery is 97% in serum (range 91–103%) and 99% in plasma (range 93–105%).

Validated sample types include serum, plasma, tissue homogenates, cell culture supernatants, and other biological fluids. The kit is intended for research use only. Alternative names for the target include 5DII, DIOII, Type 2 DI, Type-II 5'-deiodinase, ITDI2, and TXDI2, with the UniProt accession Q92813.