Human Type-1 angiotensin II receptor (AGTR1) ELISA Kit

Assay Genie offers a colourimetric Sandwich ELISA for the quantitative detection of the human Type-1 angiotensin II receptor (AGTR1). The kit is intended for use with human samples and supports research in the cardiovascular field. AGTR1 is a key receptor in the renin-angiotensin system, playing a crucial role in regulating blood pressure and fluid balance. Dysregulation of AGTR1 has been implicated in various cardiovascular diseases, including hypertension and heart failure, making it a valuable target for research and drug development.

According to the manufacturer's specifications, the assay has a detection range of 1.56-100 ng/mL and a sensitivity of 0.94 ng/mL. Reported precision values include an intra-assay CV of 5.4% and an inter-assay CV of 8.9%. The kit is specific for the natural and recombinant human Type-1 angiotensin II receptor (UniProt P30556) and is supplied for research use only. Validated sample types include serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Linearity has been characterised for serum, EDTA plasma, and heparin plasma across serial dilutions, and recovery is reported as 101% (range 95-107%) for serum and 103% (range 97-109%) for plasma. The target is a multi-pass cell membrane receptor that mediates angiotensin II signalling through association with G proteins, activating a phosphatidylinositol-calcium second messenger system.