Human TWISTNB (DNA-directed RNA polymerase I subunit RPA43) ELISA KIT (HUFI04815)

Quantification of TWISTNB, a ~43 kDa regulatory subunit of RNA Polymerase I designated RPA43, supports research into ribosomal RNA transcription, nucleolar function, and ribosome biogenesis in human cells. This Assay Genie kit delivers an immunoassay workflow on a microplate with absorbance-based readout, intended to measure TWISTNB levels in human research samples. Each kit includes a pre-coated well plate, capture and detection reagents, recombinant protein standard, assay buffers, chromogenic substrate, and stop solution, packaged in individual vials sufficient for a full plate of determinations. Reagents are shipped on cold packs and should be kept refrigerated upon arrival, with working dilutions prepared fresh before each run to preserve activity. The product is applied in studies of Pol I subunit composition, nucleolar stress responses, and cancer cell proliferation, where altered rRNA synthesis is a known driver of disease. The microplate format integrates easily with standard plate readers operating in the visible wavelength range, the workflow follows typical incubation and wash steps familiar to most immunoassay laboratories, and the product is configured for research use only.