Human TWIST1 (Twist-related protein 1) ELISA Kit (HUES03599)

Backed by Assay Genie's independent QC, this TWIST1 immunoassay delivers a quantitative dynamic range of roughly 7.8–500 pg/mL, with intra-assay CV under 10% and inter-assay CV under 12% in serum, plasma, and clarified cell lysate matrices. The pre-coated 96-well microplate uses a matched capture–detector antibody pair directed at distinct epitopes of full-length human Twist1, and the complete sandwich ELISA protocol finishes in about 3.5–4 hours when incubated at room temperature, supporting medium-throughput screening in cancer and developmental biology studies. Reconstituted standard should be single-use aliquoted and stored at -20 °C for up to 60 days, while unused strip wells remain stable inside the sealed foil pouch with desiccant for the full shelf life of six months from manufacture. The chromogenic step uses TMB substrate followed by a 2 N sulfuric acid stop solution, and absorbance is read at 450 nm with a 570 nm reference correction to subtract plate background. Researchers investigating epithelial-mesenchymal transition frequently pair this kit with companion ELISAs for E-cadherin, N-cadherin, vimentin, and Snail to map the full mesenchymal shift in head and neck squamous cell carcinoma, triple-negative breast cancer, and osteosarcoma xenograft models.