Human TWF1 ELISA Kit (HUFI05885)

The Assay Genie Human TWF1 ELISA Kit quantifies twinfilin-1, an actin monomer-binding protein that regulates cytoskeletal remodeling, cell migration, and neuronal morphogenesis. TWF1 is expressed across multiple human tissues and is investigated in hearing research, neurodevelopmental studies, and oncology contexts where altered actin dynamics contribute to disease phenotypes. The procedure uses a pre-coated microplate and incorporates a standard curve for interpolation of unknown concentrations, supporting quantitative comparison across experimental cohorts.

Each kit supplies reagents sufficient for one plate. Researchers should aliquot reconstituted material and store frozen between runs to preserve antibody activity, minimizing freeze-thaw cycles. The protocol accommodates a sample volume of 100 uL per well and is intended for research use only, not for diagnostic applications. Detection proceeds on a standard microplate reader, and results are calculated against the included standard curve.

By providing a quantitative readout of twinfilin-1 levels, the kit supports biomarker discovery workflows, mechanistic studies of actin-binding proteins, and translational investigations linking cytoskeletal regulators to cellular behavior in vitro.