Human TWEAK/TNFSF12 ELISA Kit

From Assay Genie, this sandwich ELISA kit uses a double-antibody, colorimetric format for the quantitative detection of human TWEAK protein (also known as TNFSF12) in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay targets a protein implicated in inflammation and tissue regeneration, with reported relevance to cardiovascular disease, autoimmune disorders, and cancer research.

Performance characteristics quoted by the manufacturer include a sensitivity of 18.75 pg/mL and a working range of 31.2-2000 pg/mL. Linearity across dilutions (1:2, 1:4, 1:8) is reported at 85-95% / 85-98% / 85-100% for serum, 85-93% / 87-93% / 85-95% for EDTA plasma, and 85-97% / 88-97% / 88-98% for heparin plasma. Recovery ranges are 89-101% (average 94%) in serum, 87-105% (average 94%) in EDTA plasma, and 85-100% (average 92%) in heparin plasma. Unopened kits are stored at 2-8°C for 12 months.

The protocol is a biotin-streptavidin-HRP workflow read at 450 nm after TMB development, with primary incubation at 37°C for 90 minutes, detection antibody binding for 60 minutes, and SABC binding for 30 minutes. The research area indicated for the kit is cardiovascular.