Human Tumor Susceptibility Gene 101 (TSG101) ELISA Kit

The Tumor Susceptibility Gene 101 (TSG101) protein is a 44 kDa component of the ESCRT complex, essential for multivesicular body biogenesis, viral budding (notably HIV-1 and Ebola), and cytokinesis. Aberrant TSG101 expression has been linked to breast, ovarian, colorectal, and prostate cancers, as well as to cervical and lung carcinoma progression, making quantitative detection valuable in oncology and virology research.

This Assay Genie kit ships with a pre-coated microplate, capture antibody, detection antibody, recombinant standards, and substrate reagents. The complete incubation protocol takes roughly 3 hours at 37°C and yields absorbance-based quantification on a standard plate reader. Unused strips should be sealed with desiccant and refrigerated at 4°C. Researchers commonly apply this kit to monitor TSG101 levels in cell lysates, tumor xenograft extracts, and conditioned media from virus-infected cultures.