Human Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4) ELISA Kit

OX40L (TNFSF4/CD252) is a type II transmembrane costimulatory ligand of the TNF superfamily that engages the OX40 receptor on T lymphocytes and is broadly implicated in immune checkpoint signaling, T cell expansion, and germinal center maintenance. This quantitative immunoassay from Assay Genie is supplied as a complete 96-assay kit intended for in vitro research use only, enabling laboratories to profile endogenous human TNFSF4 concentrations across serum-based samples, plasma-based samples, and tissue homogenate fractions. The procedure uses paired capture and detection antibodies specific to native human TNFSF4 and yields an absorbance-based readout compatible with standard microplate readers, supporting reproducible inter-plate comparison in routine ELISA work. Researchers apply this kit in studies of tumor microenvironment biology, autoimmune disease modeling such as systemic lupus erythematosus and rheumatoid arthritis, transplant tolerance, allergic airway inflammation, and vaccine adjuvant development, where quantitative TNFSF4 measurement helps clarify the contribution of costimulatory signaling to disease phenotypes and therapeutic response. The kit should be stored refrigerated between uses with reagents equilibrated to room temperature before opening each vial, and standard laboratory practice applies to handling of the stop solution and substrate.