Human TUBGCP4 ELISA Kit (HUFI05879)

Quantify gamma-tubulin complex protein 4 (TUBGCP4), a core scaffolding subunit of the gamma-tubulin ring complex that nucleates microtubule assembly at centrosomes, with this in vitro ELISA kit from Assay Genie. The assay targets a centrosomal protein required for mitotic spindle organization, proper bipolar spindle formation, and faithful chromosome segregation during cell division. Published loss-of-function studies link TUBGCP4 variants to primary microcephaly and neurodevelopmental defects, making the protein a relevant biomarker for cell-cycle, cytoskeletal, and neurodevelopmental research.

Built on a quantitative enzyme-linked immunosorbent format, the kit is supplied in a 96 Assays configuration intended for research use only. Standard ELISA workflow applies: prepare recombinant standards and unknown samples, load them onto the pre-coated microplate, incubate, wash, add biotinylated detection antibody followed by streptavidin-HRP conjugate, develop with chromogenic substrate, and read absorbance. Four- or five-parameter logistic curve fitting against the included standard series yields relative TUBGCP4 concentrations in each specimen.

Typical applications include profiling centrosomal and microtubule-associated protein expression in cultured human cell lines, comparing levels in proliferating versus differentiated populations, and supporting studies on spindle pole integrity, chromosome segregation fidelity, and gamma-tubulin complex composition under experimental perturbations such as RNAi knockdown, CRISPR knockout, or small-molecule inhibitors of mitotic regulators. Researchers studying centriole duplication, asymmetric division in stem cells, and congenital microcephaly mechanisms will find TUBGCP4 a useful quantitative readout.