Human TTRAP (TRAF and TNF receptor-associated protein) ELISA Kit (HUFI05886)

TTRAP, also known as tyrosyl-DNA phosphodiesterase 2 (TDP2), is a nuclear enzyme that hydrolyzes 5'-tyrosyl-DNA phosphodiester bonds, repairing covalent topoisomerase II-DNA complexes generated during replication and transcription. Investigation of TTRAP/TDP2 activity informs research on the cellular DNA damage response, resistance to topoisomerase II-targeted chemotherapeutics such as etoposide, and autosomal recessive spinocerebellar ataxia (SCAR23), a neurological condition linked to TDP2 loss-of-function mutations.

This Assay Genie ELISA kit ships as a complete package containing pre-coated microplate wells, biotinylated detection antibody, recombinant protein standards, streptavidin-HRP conjugate, TMB substrate solution, and wash buffer sufficient for 96 determinations. All components arrive in single-use aliquots supporting quantitative measurement of endogenous TTRAP in human research samples. Store unopened reagents refrigerated; protect the TMB substrate from light and equilibrate the kit to room temperature before each run. Reconstituted standards and diluted samples should be used promptly and not subjected to repeated freeze-thaw cycles.