Human Troponin I, fast skeletal muscle/TNNI2 ELISA Kit

A sandwich ELISA kit from Assay Genie designed for the quantitative measurement of human Troponin I, fast skeletal muscle (TNNI2) in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay uses a double-antibody sandwich format with a colorimetric readout measured at 450 nm.

Performance specifications include a sensitivity of 46.875 pg/mL and a working range of 78.125–5000 pg/mL. The protocol begins by equilibrating reagents and the TMB substrate to room temperature, followed by a 90-minute primary incubation at 37°C for antigen binding, a 60-minute biotin-labeled detection antibody step, a 30-minute HRP-Streptavidin (SABC) incubation, and 10–20 minutes of TMB colour development in the dark. The reaction is stopped and absorbance read at 450 nm. Reagents are stored at 2–8°C for up to 12 months.

Validated linearity at 1:2, 1:4, and 1:8 dilutions is reported for serum (86–100%, 83–101%, 80–97%), EDTA plasma (85–105%, 82–100%, 84–94%) and heparin plasma (91–105%, 85–99%, 84–100%). Recovery ranges across the same sample types span 87–104%, with average recoveries of 95–98%. TNNI2 is a fast skeletal muscle protein that contributes to muscle contraction, and abnormal levels have been associated with muscle disorders, supporting its use as a biomarker in muscle-related research.