Human TRNA Methyltransferase 61B ELISA Kit

This quantitative immunoassay from Assay Genie enables researchers to measure TRNA Methyltransferase 61B (TRMT61B) protein levels in human-derived samples. TRMT61B is a mitochondria-localized methyltransferase that installs N1-methyladenosine (m1A) at position 58 of mitochondrial tRNAs, a modification essential for proper mitochondrial translation. Dysregulation of TRMT61B has been implicated in published studies of mitochondrial disorders, cancer metabolism, and ribosomal stress responses, making the target relevant to multiple investigative fields.

The kit supports research into the role of tRNA epitranscriptomic modifications in cellular homeostasis, providing a tool to quantify enzyme abundance across experimental conditions. Researchers examining the crosstalk between nuclear-encoded mitochondrial enzymes and respiratory chain function, or profiling TRMT61B expression in tumor versus matched normal tissue, can integrate the assay into their workflows. Reagents are supplied in individual vials for reconstitution as outlined in the assay protocol, and the kit is intended for in vitro laboratory applications.

For research use only; not intended for diagnostic or therapeutic procedures. Downstream validation approaches such as Western blot, cell culture perturbation experiments, and tissue homogenate preparations complement the quantitative read-out provided by this ELISA-based workflow.