Human TrkB/NTRK2 ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA in a double-antibody format is designed for the quantitative measurement of TRKB levels in human samples. The assay is reactive with human specimens and is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Detection is colorimetric, with absorbance measured at 450 nm after addition of the stop solution. The kit has a stated sensitivity of 56.25 pg/mL and a working range of 93.75-6000 pg/mL.

TRKB, also known as neurotrophic receptor tyrosine kinase 2, is a receptor involved in neural development and function, contributing to neuronal survival, differentiation, and plasticity. The Human TrkB/NTRK2 ELISA Kit is intended for research applications relating to this receptor, including studies of neurological disorders and conditions such as Alzheimer's disease and depression. Uniprot reference: Q16620.

Validation data from the manufacturer include linearity in serum, EDTA plasma, and heparin plasma across 1:2, 1:4, and 1:8 dilutions. Serum linearity ranges from 88-105% at 1:2 to 91-101% at 1:8. EDTA plasma ranges from 89-98% at 1:2 to 83-100% at 1:8, and heparin plasma ranges from 80-100% at 1:2 to 81-99% at 1:8. Recovery data show serum averaging 95% (range 89-104%), EDTA plasma averaging 96% (range 87-104%), and heparin plasma averaging 94% (range 85-103%). Kit components are intended to be stored at 2-8°C for 12 months, with the microplate and lyophilized standard also storable at -20°C for 12 months.