Human Triosephosphate isomerase/TPI1 ELISA Kit

From Assay Genie, this sandwich ELISA kit is designed for the precise measurement of triosephosphate isomerase (TPI1) levels in human samples, including serum, plasma, cell culture supernatants, cell or tissue lysates, and other liquid samples. The double-antibody assay format delivers high specificity and uses a colorimetric detection method.

TPI1 is a key enzyme in glycolysis and plays a critical role in energy production. Dysregulation of TPI1 has been linked to metabolic disorders and cancer, making it a valuable biomarker for studying these conditions and for potential therapeutic interventions. The kit is suited to the metabolism research area and reports a sensitivity of 0.469 ng/mL with an assay range of 0.781-50 ng/mL. It is intended for storage at 2-8°C for 12 months.

Linearity in serum, EDTA plasma, and heparin plasma (n = 5) is demonstrated across 1:2, 1:4, and 1:8 dilutions, while recovery ranges from 86% to 105% with averages of 96% (serum), 90% (EDTA plasma), and 95% (heparin plasma). The protocol uses a biotin-labeled detection antibody and HRP-Streptavidin binding, with TMB substrate development and absorbance read at 450 nm.