Human TRIM5 (Tripartite motif-containing protein 5) ELISA Kit (HUFI05952)

Tripartite motif-containing protein 5 (TRIM5) is a ~57 kDa cytoplasmic restriction factor central to innate antiviral immunity, where it recognizes incoming retroviral capsids and blocks early stages of viral replication. The Assay Genie TRIM5 ELISA Kit gives researchers a quantitative tool to measure TRIM5 levels in experimental samples and characterize expression under varying conditions.

This research use only kit supports 96 determinations and ships with the pre-coated microplate and reagents needed to run the full assay. Typical applications include studies of host-pathogen interactions, innate immune signaling, lentiviral tropism in cell culture, and comparative analysis of restriction factor activity across cell types and experimental treatments.

Standard workflow: add samples and standards to the plate, incubate, wash, develop with substrate, and read the wells. Investigators should aliquot reagents upon receipt, protect from light where indicated, and follow the supplied protocol for storage temperature and handling of the conjugate, wash buffer, and stop solution.