Human TRIM33 (tripartite motif-containing 33) ELISA Kit (HUFI05965)

Built for quantitative measurement of tripartite motif-containing 33 (TIF1-gamma) in human specimens, the Assay Genie Human TRIM33 ELISA Kit uses a colorimetric signal readout to detect this RING-type E3 ubiquitin ligase. TRIM33 operates within the TGF-beta signaling axis, where it modulates SMAD4 activity and influences transcription factor turnover. Researchers studying hematopoiesis, myeloid differentiation, and leukemia use TRIM33 abundance as a biomarker for pathway activity and as a readout in CRISPR or RNAi screens targeting chromatin regulators.

The procedure accepts serum or plasma preparations alongside cell lysate and tissue homogenate samples, supporting studies of how TRIM33 levels reflect disease status in oncology, fibrosis, and immune regulation. Each kit contains reagents sufficient for 96 Assays, with all buffers, standards, and detection components required to generate a standard curve and quantify endogenous TRIM33. The workflow follows a standard incubation-and-wash protocol that produces an optical density proportional to TRIM33 concentration in the specimen.

For research use only; not intended for diagnostic procedures.