Human Tribbles homolog 3 (TRIB3) ELISA Kit

From Assay Genie, this Sandwich ELISA kit is designed for the quantitative measurement of human Tribbles homolog 3 (TRIB3) in serum, plasma, tissue homogenates, cell culture supernatants, and other biological fluids. It uses a colorimetric detection method and is reactive with human samples.

TRIB3 is a key regulatory protein involved in apoptosis, autophagy, and insulin signaling, and its dysregulation has been implicated in cancer, diabetes, and cardiovascular disorders, making it a valuable biomarker for disease research. The kit provides a detection range of 0.156–10ng/mL with a sensitivity of 0.071ng/mL, and reports an intra-assay CV of 6.3% and inter-assay CV of 10.1%. It is specific for natural and recombinant human Tribbles homolog 3, is intended for research use only, and supports applications in cancer research, with a subcellular localisation of the nucleus.

Known synonyms for the target include Neuronal cell death-inducible putative kinase, SINK, p65-interacting inhibitor of NF-kappa-B, TRB-3, C20orf97, NIPK, SKIP3, and TRB3. Functionally, the protein disrupts insulin signalling by binding directly to Akt kinases and blocking their activation, and interacts with AKT1, AKT2, ATF4, MAP2K1, MAP2K7, DDIT3/CHOP, APOBEC3A, and APOBEC3C.