Human TRDMT1 (tRNA (cytosine-5-)-methyltransferase) ELISA KIT (HUFI04809)

Quantitative measurement of human tRNA methyltransferase activity is supported by this 96 Assays immunoassay kit from Assay Genie. The TRDMT1 enzyme, also referred to as DNMT2 in published literature, catalyzes methylation at cytosine-5 residues of specific tRNA molecules and contributes to epitranscriptomic gene regulation, stress granule formation, and cellular responses to oxidative damage.

Each kit contains a pre-coated well plate, detection antibody, standard calibrators, substrate solution, stop reagent, wash buffer concentrate, and a printed protocol booklet. Reagents should be stored refrigerated between runs; protect from light and avoid repeated freeze-thaw cycles to preserve activity. Aliquot the detection antibody upon first thaw to maintain consistent performance across multiple experiments.

Investigators studying tRNA modification biology, cancer epigenetics, hematopoietic differentiation, or comparative enzyme expression work can integrate this kit into standard ELISA workflows. The single-use well plate configuration supports reproducible standard curves, and the included protocol walks users through sample preparation, incubation timing, and data analysis recommendations suitable for cell culture supernatants, tissue homogenates, and other research specimens.