Human Transportin 2/TNPO2 ELISA Kit

From Assay Genie, this Human Transportin 2/TNPO2 ELISA Kit is a sandwich ELISA designed for the quantitative measurement of Transportin-2 in human samples. The assay uses a colorimetric detection method and is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Transportin-2, also known as karyopherin beta-2, is a key protein involved in the nucleocytoplasmic transport of various macromolecules including RNA, and its dysregulation has been implicated in cancer, neurodegenerative disorders, and viral infections.

The kit has a stated sensitivity of 0.188 ng/mL and an assay range of 0.313-20 ng/mL. Kit components are stored at 2-8°C for 12 months, with the lyophilized standard and microplate strips also suitable for 12-month storage at -20°C. Reported recovery performance includes serum averaging 98% (range 95-102%), EDTA plasma averaging 93% (range 89-103%), and heparin plasma averaging 91% (range 85-99%). Linearity across 1:2 to 1:8 dilutions has been demonstrated for serum (91-103%), EDTA plasma (85-100%), and heparin plasma (80-102%).

The protocol involves primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody incubation at 37°C for 60 minutes, HRP-Streptavidin binding for 30 minutes, TMB substrate development in the dark for 10-20 minutes, and absorbance reading at 450 nm immediately after addition of stop solution.