Human Transmembrane Protease,Serine 6 (TMPRSS6) ELISA Kit

Matriptase-2, the protein product of the TMPRSS6 gene, is a hepatic type II transmembrane serine protease that suppresses hepcidin expression and controls systemic iron homeostasis. Loss-of-function mutations in TMPRSS6 cause iron-refractory iron deficiency anemia (IRIDA), making accurate protein-level measurement a useful tool in hematology and metabolic disease research. The TMPRSS6 ELISA Kit from Assay Genie is configured for quantitative detection of this protease in human samples.

Validated reactivity covers tissue homogenates and cell lysates, and the assay uses a colorimetric method read on a standard microplate reader. Each kit ships with pre-coated microplate strips, biotinylated detection antibody, calibrated protein standards, wash buffer, and substrate solution in individual vials, with reagents sufficient for 96 determinations. Refrigerate kit components upon arrival, and aliquot standards and antibody into smaller volumes to limit freeze-thaw cycles during long-term projects.

Typical laboratory applications include profiling TMPRSS6 expression in hepatic cell models, characterizing iron overload and deficiency phenotypes, mapping hepcidin signaling pathways, and screening RNAi or pharmacological tools that modulate protease activity in vitro. The kit is intended for research use only and is not validated for clinical or diagnostic decision-making.