Human Transglutaminase 3, Epidermal (TGM3) ELISA Kit

Epidermal Transglutaminase (TGM3) is a calcium-dependent cross-linking enzyme that operates during formation of the cornified cell envelope, the protective barrier that seals the outermost layers of the skin. This Assay Genie kit delivers a quantitative immunoassay workflow for measuring human TGM3 protein in cell lysates and tissue homogenates prepared from experimental skin, mucosal, and epithelial cell models. TGM3 expression is a target of interest in studies of dermatitis herpetiformis, psoriasis, atopic dermatitis, and several epithelial cancers where altered keratinization has been reported in published dermatology research. The product ships as a complete 96-assay kit with a pre-coated microplate, reference standard, detection reagents, and substrate buffer sufficient for a full standard curve plus replicate unknowns. The protocol follows a standard research ELISA workflow compatible with the microplate readers routinely used in cell culture and biochemistry laboratories. For research use only, and not intended for clinical diagnostic procedures. Reagents should be stored as indicated in the supplied manual to preserve activity through the stated shelf life.